The effect of intravenous injection of kit + cells on the expression of FGF2, HSPGs and fibrosis in the heart tissue of type 2 non-diabetic rats

Abstract

Cardiac fibrosis as a secondary complication of diabetes leads to systolic and diastolic heart dysfunction. The aim of this study was to evaluate the effect of intravenous injection of c-kit + cells on the expression of FGF2, HSPGs and fibrosis in the heart tissue of type 2 non-diabetic rats. method: 58 male Wistar rats were randomly divided into 4 groups: control (n = 12), diabetic (n = 12), diabetic + c-kit (n = 12) and diabetic + c-kit ( n = 12) were divided. Diabetic groups developed diabetes by receiving a high-fat diet and intraperitoneal injection of streptozotocin (35 mg / kg). Diabetic group 50 μl normal saline solution and diabetic group + c-kit cell, 50 μl normal saline solution containing 1 million ⁺ c-kit cells and diabetic group + c-kit cell, 50 μl normal saline solution containing 1 million ¯ c-kit cells intravenously they received. Finally, 60 days later (cardiac changes), the animals were anesthetized with ketamine and xylazine and immediately the heart (Apex) was removed for histological studies and the rest of the left ventricle was measured for expression of FGF2 and HSPGs (Glypican1 and SYNDECAN4) as fibrotic markers. The one-way ANOVA test was analyzed. Results The results of this study showed that the expression of Glypican1 and SYNDECAN 4 genes in all diabetic groups had a significant increase compared to the control group, but the values of these genes in the C-KIT + group showed a significant decrease compared to the diabetic group. . FGF2 gene levels decreased in diabetic groups (groups D and -C-KIT) but FGF2 levels in C-KIT + group showed a significant increase. Also, histological study showed fibrotic changes in the left ventricle.