Evaluation of microRNA expression levels of miR-27a, miR-124a, miR-133b, miR-212, miR-125, involved in carcinogenesis in tumor and marginal specimens in patients with laryngeal cancer

Abstract

Laryngeal cancer is one of the main cancers of the head and neck that if not treated and diagnosed in time can lead to death. Both environmental and hereditary factors play an important role in the development of this disease, while the role of environmental factors such as smoking is much more colorful than genetic factors. Given the low prevalence of this disease compared to other common malignancies, little focus is on the pathogenesis of this disease, but new efforts and studies have considered the role of different genes, including the gene group of micro-RNAs in causing this disease. Micro RNAs are among the most important genes involved in the development of various cancers. In this regard, in the present study, changes in the relative expression levels of some of these genes were considered to better understand the gene pathways involved in this disease.

Materials and Methods: During the surgical procedure of laryngeal cancer patients, a collection of 20 tumor samples and appropriate healthy margins were collected. After total RNA extraction, real-time PCR was used to measure changes in gene expression. Pvalue <0.05 was considered significant in statistical tests.

Results: After the studies performed between the studied genes, the relative expression level of all micro-RNAs studied in this study has significant changes between the two groups