The effect of swimming training on autophagy-related genes in kidney tissue of type-1 diabetic mice

Abstract

In recent years, defective autophagy has been shown to be a major cause of kidney tissue damage in diabetes. Therefore, in this study, the effect of swimming training on the expression of autophagy-related genes in the kidney of type -1 diabetic mice was investigated. Methods 32 adult male mice weighing 25±30 g were randomly divided into 4 groups (each in 8) as follows: Control group (C), Swimming group (Swimming), Diabetic group (D) and Diabetic + Swimming group (D+ Swimming). In diabetic groups, type 1 diabetes was induced by intraperitoneal injection of a single dose of streptozotocin (50 mg / kg). Two weeks ofter induction of diabetes, swimming training was performed for four weeks. Forty-eight hours after the last swimming training, the animals were killed and their kidney tissue was removed. Pathological changes were monitored using H&E staining. The expression of related genes was measured using real-time PCR assay. Results Data showed pathological changes in kidney tissues of diabetic mice coincided with up-regulation of IL-1β and TNF-α and down-regulation of Becline-1, LC3 and P62 compared to the control group. swimming training improved the levels of these parameters. No statistically significant differences were not obtained in Swimming group in comparison with control group.