Fatty acids of type 2 diabetic serum decrease the stemness properties of human adipose derived mesenchymal stem cells

Abstract

Diabetes mellitus (DM) is caused by deficient insulin secretion or insulin resistance leading to dyslipidemia. Human adipose tissue-derived mesenchymal stem cells (AdMSCs) are crucial for the regeneration process of adult tissues. Since regenerative capacity is impaired in DM, this study aimed to evaluate the effect of diabetic serum free fatty acids (FFAs) on stemness of AdMSCs in vitro.

Methods AdMSCs were retrieved from abdominal adipose tissue of young, healthy individuals. Pooled diabetic and non-diabetic serums were then prepared from 7 and 8 women in menopausal ages (up to 65 year), respectively. AdMSCs were treated with Whole diabetic serum (DS) or non diabetic serum (nDS), lipid depleted DS and lipid depleted nDS, FFA enriched serums (nDS FFA and DS FFA) and pure OA. FFA composition was analyzed using gas-liquid chromatography. The expression of stemness markers CD49e and CD90 were also analyzed by quantitative PCR. Cell proliferation was evaluated by BrdU assay. The study was approved by the local ethics committee (No. IR.TBZMED.REC.1398.659).

Results A significantly higher oleate level was observed in DS (1.4-fold, p<0.05) compared to NDS. Compared to NDS, DS significantly decreased CD49e expression (0.4±0.24 versus 1.05±0.43-fold change, p<0.05, respectively). However, no significant differences were observed in CD90 expression between the groups. Compared to NDFS, DFS significantly decreased the CD49e and CD90 expression (4.14±0.08 versus 6.2±0.46-fold change, p<0.05, and 1.34±0.17 versus 2.74±0.5, p<0.05, respectively). Additionally, DFS significantly decreased AdMSCs proliferation rate in contrast to NDFS (68.0%±1.0 versus 105.36%±0.65, p<0.05).