Synthesis of acrylic-type magnetic nanogels conjugated with As1411 aptamer for delivery of Erlotinib on prostate cancer cells

Abstract

Introduction: Prostate cancer (PC) is a common malignancy that is generally slower than other cancers and is an important cause of death among men. Aims: The present study expanded the magnetic pH-dependent, and nucleolin receptor-targeted nano-drug delivery system consists of synthesized superparamagnetic iron oxide nanoparticles (SPION), poly N-isopropyl acrylamide (PNIPAM) with aptamer AS1411 to deliver the erlotinib (ERL) to prostate cancer (PC) cells. Methods: In this project, N-isopropyl acrylamide polymer (NIPAAm) was polymerized in the presence of polyethylene glycol diacrylate as a cross-linker by co-deposition polymerization with galium per soulfat as initiator in water solvent and synthesized on Fe3O4 magnetic nanoparticles. The resulting polymer will be used to prepare nanoparticles containing Erlotinib by W / O emulsion method. Aptamer As1411 is then conjugated to this nanoparticle by EDC / NHS. The physicochemical properties of the nanoparticles such as drug loading interest, particle size and morphology, and how the drug is released from the polymer will be investigated. The cytotoxicity of nano-particles on prostate cancer cell line DU-145 will be studied by MTTassay method. Results: The properties of formulated nanoparticles were determined using different techniques. The encapsulation (EE) and drug loading (DL) efficiencies were about 34% and 85%, respectively. Characterization studies showed that the magnetic nanoparticles are spherically formulated with an average particle size of 97.8 nm, a PDI of 0.03 and a zeta potential of 2 mV. Conclusion: The results showed that synthesized polymer magnetic nanoparticles, while increasing the amount of drug loading, improved the effectiveness of the erlotinib in prostate cancer cells.