Evaluation of the preventive effect of Artemisia marshalliana plant extract and vitamin C on vincristine-induced neuropathy in mice

Abstract

Introduction:Various mechanisms are involved in vincristine neuropathy including the activation of oxidative stress - increasing the activity of NMDA and serotonin receptors etc. Vitamin C and Artemisia marshalliana are among the agents with antioxidant properties. Objective: The aim of this study is to investigate the preventive effect of vitamin C and Artemisia marshalliana extract on neuropathic pain caused by vincristine injection in mice.Goal: The aim of this study is to investigate the preventive effect of vitamin C and Artemisia marshalliana extract on neuropathic pain caused by vincristine injection in mice. Materials and methods: 72 male mice in the weight range of 25-35 grams were randomly divided into 9 groups of 8 and were subjected to the following treatment regimens for 25 days. Different doses of AM and VITC were injected three days before vincristine injection. And on the fourth day, vincristine was administered as a single dose. Next, 3 days of drug injection and 3 days of rest were considered. The next day the hot plate test was done. This 7-day process was repeated 3 times in all groups. At the end of the 25th day, blood was taken from the heart of animals to perform MDA and TAC tests Result: The results indicated a significant effect of different doses of vitamin C (IP, 100, 50, 25 mg/kg) and A.M extract (IP, 5, 10, 20 mg/kg). Also, a significant effect of vitamin C and A.M extract in doses (IP, 100mg/kg, 50, 25) was observed in reducing MDA level and increasing TAC. In the combined injection of vitamin C and A.M extract, a better effect was observed than the injection of these two drugs alone in preventing neuropathy. Conclusion: Vitamin C and A.M extract have beneficial effects in preventing neuropathy caused by vincristine, additional studies in humans are needed..