The effects of hormone therapy on oocyte maturation associated genes and apoptosis incidence in ovarian tissue of polycystice ovary syndrom model mice

Abstract

"Polycystic ovary syndrome" is a general disorder of the endocrine system in women of childbearing age. The aim of this study was the effects of hormone therapy on genes associated with oocyte maturation and the incidence of apoptosis in the ovaries of polycystic ovary model mice. Materials and Methods: In this experimental study, 48 female Wistar rats were used and randomly divided into six groups: Group 1: Normal control, Group 2: Control with PCOS; Group 3: Combined polycystic ovary group By injecting one unit of HCG in the estrous phase; Group 4: Polycystic ovary group with injection of one FSH unit in estrous phase; Group 5: Polycystic ovary group with injection (FSH (1U) + LH (1U in estrous phase); Group 6: Polycystic ovary group with injection of one unit of estrogen in PCOS estrose phase with a dose of "subcutaneous injection of estradiol valerate" ( 16 mg / kg. After the treatment period, the animals were anesthetized, then their plasma samples were used for hormonal examination. The ovaries of mice were removed and evaluated for histopathology as well as expression of genes related to folliculogenesis in ovarian tissue. evaluated. Results: The plasma MDA level in PCOS group increased significantly (P <0.05) and decreased significantly in treatment groups (P <0.05). In addition, serum levels of SOD and GPX in PCOS decreased significantly (P <0.05), while in animals in the treatment group, its level increased significantly (P <0.05). In addition, damage was observed in the ovarian tissue of the PCOS group. While in the treated groups this change improved. Expression of genes associated with oocyte maturation was significantly reduced in PCOS group and this reduction was significantly compensated in treatment groups.