The protective effect of hydroalcholic extract of orange peel on the expression of PCNA and FSH-R genes and histological damage and oxidative stress caused by ovarian torsion in adult rats

Abstract

Ovarian torsion/detorsion or ischemia / reperfusion is a pathophysiological event. Because histological damage from ovarian torsion is observed with decreased blood flow and subsequent lack of oxygen (ischemia) in the ovarian tissue, the present study aims to investigate the protective effect of hydroalcholic extract of orange peel on PCNA and FSH-R gene expression in Histological lesions and oxidative stress caused by ovarian torsion in adult rats were performed. Research Method: For this purpose, 32 adult female rats were randomly divided into 4 groups. Group 1: Sham (incision of the abdominal wall without torsion) Group II: Hydro alcohol extract of orange peel prepared will be added to their diet for two weeks. For the third group (control group), only the standard diet will continue. After about 2 weeks, ovarian torsion will be performed with 2 hours of ischemia and 2 hours of reperfusion. Ovarian torsion for two hours and ovarian torsion for 2 hours. Ovarian resection is then performed to assess histological lesions and expression of PCNA and FSH-R genes and blood sampling to assess serum levels of antioxidant enzymes. Results: Histological, gene expression, biochemical and hormonal studies showed that torsion and detoxification have destructive effects on the ovarian tissue of mice, and the administration of hydroalcoholic extract of orange peel due to its high antioxidant properties improves these effects