Determination of HOTAIRM1 Non-coding RNA Expression and its Relation to Clinical Pathological Findings in Breast Cancer Tumor Specimens

Abstract

Breast cancer is a common human malignancy with a high mortality rate worldwide. Recent studies have been focused on the understanding the involvement of long non-coding RNA in the pathogenesis of breast cancer. Therefore, the present study aimed to measure the expression of HOTAIRM1 in tumor tissue and adjacent normal tissues in patients with breast cancer and its relationship with clinicopathological factors. Methods The expression levels of lncRNA HOTAIRM1 were measured in the tumor tissues and adjacent normal tissues of sixty patients with breast cancer using quantitative real-time polymerase chain reaction (qRT-PCR). Finally, the relationship between this gene's levels and clinicopathological factors between the two statistical tissues of tumor and healthy was compared. Results Our results showed that the expression levels of HOTAIRM1 were significantly higher in breast tumor tissue in comparison to normal tissues (P<0.05). in addition, the expression levels of HOTAIRM1 in tumor tissues were strongly correlated with patients age, tumor size, cancer grade and lymph node metastasis.