Effects of probiotic yeast, Saccharomyces cerevisiae and Saccharomyces boulardii on proliferation and apoptosis of breast cancer cells (MDA-MB-231)

Abstract

Probiotics are living, non-pathogenic microorganisms found in some foods that, when taken in sufficient amounts, have a positive effect on host health. Today, probiotics are recognized as a factor in preventing many infectious and cancerous diseases. The anti-carcinogenic properties of probiotics are achieved by neutralizing the toxicity of substances that cause gene damage. Materials and Methods: In this study, breast cancer cells were first cultured in RPMI-1640 culture medium containing 10% FCS, under 5% CO2 and at 37 ° C. The cultured yeasts were then isolated using cell wall sonication. MTT test was used to evaluate the inhibition of growth and proliferation of cancer cells. The migration and metastasis of cancer cells after tritus were also examined by the Wound healing assay technique. Flow cytometry with Annexin V and PI was used to evaluate the occurrence of apoptosis in cells. The expression levels of K-Ras, MMP9, Caspase-3, Bax, Caspase-9, Bcl2 and c-Myc genes were evaluated by yeast metabolite in cancer cells by qRT-PCR. Results: BAX gene expression was significantly increased in treat with Bolardi sacromysez and Sacromyces cerevisiae. BCL-2 gene expression was significantly reduced in the group containing cerevisiae sucrose yeast. Expression of Cas3 gene in treat with Saccharomyces cerevisiae was significantly increased compared to the group that did not receive treatment. Expression of Cas3 gene in treat with Saccharomyces cerevisiae was significantly increased compared to the group that did not receive treatment. There was a significant increase in treatment with Bolardi sucromycosis yeast. Also, treatment with Bolardi sucromycosis yeast caused a significant increase in CAS 9 gene expression, which is greater in treat with bulardi yeast and increased use of the combination of these two yeasts. Gene expression is higher than when one type of yeast is used alone. C-Myce gene expression was significantly reduced in treat by combining these two yeasts. Gene expression was greater than that of treat alone. Treat with Saccharomyces cerevisiae and Bolardi sucromycosis yeast significantly reduced K-Ras gene expression, which decreased. Gene expression in treat by combining these two yeasts is higher than treatment to yeasts alone. Expression of MMP9 gene in treatment by combining these two yeasts was significantly reduced and this reduction was greater than treat with Saccharomyces cerevisiae alone.