Preparation and study of physicochemical characteristics of chitosan-alginate containing atorvastatin2

Abstract

Introduction: Recently, statins have attracted great attention among drugs used in bone repair. Statins are well-known lipid-lowering drugs. The pleiotropic effects of statins have brought about some beneficial effects on improving the therapeutic outcomes of cell therapy and tissue engineering approaches. The potential of this statins loaded hydrogel to improve bone regeneration and to decrease soft tissue inflammation. Statins have a significant effect on bone cell differentiation. Many mediators of osteogenesis, including BMP-2, are directly influenced by statins. Therefore, monitoring the release of Atorvastatin via hydrogel designed based on chitosan-tripolyphosphate-alginate was evaluated. Goal: In this study we aim to Preparation and study physicochemical characteristics of Cross-linked CS-TPP-ALG hydrogels containing atorvastatin and assessment release profiles atorvastatin over a period of time. Methods: In this study Cross-linked CS-TPP-ALG hydrogels containing atorvastatin (ATR) prepared via ionic gelation interaction in order to achieve a sustained release of Atorvastatin. Physicochemical characteristics of CS-TPP-ALG-ATR such as size, morphology and swelling were studied. The Hydrogel morphology and functional group were evaluated by SEM and FTIR, respectively. Results: Chitosan alginate hydrogel was prepared in different ratios with a certain percentage of TPP cross linker and loaded with atorvastatin. The drug is loaded with an interest rate of nearly 100%. The results showed that in the first hour, an explosive release of about 25% is observed and then the drug is gradually released in about 35% for ten hours. The percentage of cross linker has a large effect on encapsulation gain and release profile. Discussion: Chitosan alginate cross-link hydrogel can be a suitable scaffold for loading all kinds of drugs and macromolecules and cells due to its high swelling percentage and high porosity. By controlling the ratio of polymers and the cross-linker ratio, the percentage of drug loading and release can be controlled.