Evaluation of the effect of using polyethylene glycol precipitation method on the efficiency of separation and purification of IVIG from cryo poor plasma
چکیده
Introduction: Human plasma is a source of various antibodies and proteins and is the source of a wide range of life-saving drugs for patients. Intravenous immunoglobulin (IVIG) is used effectively in the treatment of disorders such as primary and secondary immunodeficiency, autoimmune, inflammatory systemic and also in infectious diseases and is currently one of the most widely used plasma components in the world.
Objective: Due to the therapeutic applications of IVIG, achieving the highest efficiency and purity of these antibodies through a simple, repeatable and inexpensive method is very important . . . . . . . . ..
Methods: Cryo poor plasma was used as the source of IVIG. The effect of different percentages (w/v) of polyethylene glycols of 4000, 6000, 8000 and 10000 Dalton and pH values of 6.5, 7 and 7.4 in IVIG separation was investigated by polyethylene glycol (PEG) precipitation method. In the final stage, the optimised paraeters including (pH, molecular weight of polyethylene glycol and its percentage) were applied were reprted as the best effective conditions in separating IVIG from other proteins in CPP. The precipitation pattern of proteins, especially IVIG, was analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE).
Conclusion: The results showed that the best pH and mass of polyethylene glycol in IVIG precipitation were 35% of 4000 dalton polyethylene glycol at pH 7.4, 15% of 6000 dalton polyethylene glycol at pH 7, 20% of 8000 dalton poly Ethylene glycol at pH 6.5, and 25% of 10000 dalton polyethylene glycol at pH 6.5.