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Evaluation of antibacterial effect of Origanum vulgare extract , Zingiber officinale essential oil , 5.25% sodium hypochlorite and 2% chlorhexidine as root canal irrigants againts 6-weeks-old Enterococcus faecalis biofilm at the root of the human permanent teeth(An invitro study)

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sepideh alizadeh thesis.pdf (2.420Mb)
Date
2021
Author
Alizadeh, Sepideh
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Abstract
Introduction: Successful root canal treatment requires shaping and cleaning of root canal systems. One of the most important goal of root canal therapy is elimination of microorganisms and their toxins and smear layer in the root. Endodontic infections are polymicrobial isolated microorganism. Enterococcus faecalis is the most frequently found microorganism in root canal fail treatment. Role in disinfection of root canal system along with mechanical instrumentation to be key to successful endodontic treatment. The present study was aimed to compare the antibacterial of Origanum vulgare extract, Zingiber officinal essential oil , 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine ( chx) as root canal irrigants againts 6 week-old Enterococcus faecalis biofilm by the use of colony counting assay. Method and materials: In this in vitro study 66 extracted human single canal teeth were collected. Teeth having defects such as abnormalities and caries, calcification, trauma , Internal and external resorption and sever root curve excluded from this study. All sample canals manually prepared and working lengths of the teeth determined up to file number 40#. Enterococcus faecalis was inserted into the canals and replaced daily with new culture medium to produce biofilm within 6 weeks. Then 2 random samples were examined for confirmation of biofilm formation in tubuls under electron microscopy (SEM). The sample randomly divided into six groups ,each group consisted of 11 root canals. samples were wash experimental solution and essential oil in the corresponding groups. The root canals in group 1: were washed by oregano extract , group 2 irrigated by ginger oil and in group 3 canals were washed by 5.25% NaOCl , and group 4 irrigated by 2% chx and no irrigation use in positive control group. Then each of the dentin chips and debries collected was dissolved in certain volume of normal sterial saline and cultured in plate containing solid culture medium for bacterial count (CFU) and finally the colony count machine determined the number of bacteria in each sample at the end of the study. Data was analyezed using spss17 software and comparasions between the different variables were conducted through the kruskal wallis and applying mann whitney tests with the level of significance set at p≤ 0.05. Results: 5.25 % NaOCl and 2% chx were the most effective irrigants in the elimination of E.faecalis , and Zingiber officinale essential oil was less effective than Oregano vulgaris extract compare with the possitive control group. Chlorhexidine , sodium hypochlorite , Zingiber officinale essential oil and Oreganum vulgare extract had 100 % , 100 % , 99.36 % and 99.28% antimicrobial effects respectively . All four groups were significantly different from the positive control group but were not significantly different from each other (p˃0/05). Conclusion: Considerring the limitation of this study synthentic medicaments 2% chlorhexidine and 5.25% sodium hypochlorite achieved 100% growth inhibitory effect. However all 4 groups showed significant antibacterial activity compare to the possitive control group , so chlorhexidine and sodium hypochlorite had the greatest effect , followed by Zingiber officinal essential oil and Origanum vulgare extract. The herbal were effective against E.faecalis in dentine of human teeth. The use of these herbal alternative as a root canal irrigant might prove to be advantageous considering the several undesirable characteristics of synthetic medicaments.
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http://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/65273
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