Evaluation of attachment and proliferation of dental pulp stem cells on scaffolds containing nano-hydroxyapatite and MTA

Abstract

Introduction: Pulp capping is one of the treatment methods in restorative dentistry which is done in two direct and indirect methods. Despite advances in pulp biology, there is no single direct pulp capping method that is predictable and reliable that preserves pulp life and function.

Due to the positive and significant results in the application of nanohydroxyapatite and MTA in strengthening of mineralized structures, this study sought to introduce a new dental material used in direct pulp capping.

In this study, compatibility, proliferation and adhesion of dental pulp stem cells seeded on scaffolds containing nanohydroxyapatite and MTA were investigated.

Materials and Methods:

First, Scaffolds synthesis was performed by factorizing nanocrystalline cellulose and chitosan by combining a certain percentage of MTA and HAP according to previous studies of this team. Stro1 + dental pulp stem cells were cultured in DMEM and FBS 10% and 1% AB penicillin streptomycin in a 75 cc flask and attached to scaffolds after disinfection. Cell proliferation study was performed on days 1,3,5 by MTT assay method. For this purpose, a 96 well plate was considered for each day.

A 6-well plate was considered for cell adhesion checking by electron microscopy.

Independent t-test or its non-parametric equivalent and Mann-Whitney test were used to compare the proliferation and adhesion of dental pulp cells in the two groups.

SPSS17 software was used for data analysis. A probability value of less than 0.05 was considered as a significant level.

Results

According to the results of this study, tooth pulp stem cells cultured on scaffolds containing MTA and nanohydroxyapatite have high proliferative power and in the vicinity of these scaffolds, toxic and toxic properties of scaffolds were not observed. This cell proliferation on days 1, 3 and 5 was higher than the control group without Scofeld and on days 3 and 5 in the Scaffold group containing MTA and nHAP was significantly higher than the Scaffold group without stimulus.

The results show the positive effect of scaffolds containing MTA and nanohydroxyapatite on cell proliferation and non-toxicity. SEM images also show cell adhesion and the formation of cell colonies.

Conclusion: Therefore, due to the mineralogenic properties of these two compounds and their safety, it seems that this scaffold can be used as an effective compound in the direct pulp cap and probabely regeneration of tooth and bone tissues in future.