Isolation and identification of methanolic extracts of Teucrium orientale aerial organ

Abstract

Introduction: Being a member of the Lamiaceae family, Teucrium orientale (named Maryam Nokhodi in Iran) grows in Iran, Turkey, Iraq, Caucasus, Syria, Lebanon, Afghanistan, Pakistan, Turkmenistan, Central Asia, Europe, and North Africa. The genus Teucrium has about 200 species that 12 of them are present in Iran and among them, one is Teucrium orientale. This plant has shown a variety of pharmacological effects. Among the pharmacological effects, Teucrium orientale is confirmed to have antidiabetic, antihemorrhoidal, anticancer, antioxidant, antibacterial and many other effects. The genus Teucrium comprises many bioactive compounds, including flavonoids, monoterpenes, sesquiterpenes, diterpenoids, phenolic acids, and fatty acid esters. In Iranian folk medicine this plant is used as a stimulant, tonic, sudorific, appetizer, and for treatment of some digestive aliments and diabetes. Numerous studies have been reported on the secondary compounds of this plant from different countries, but studies conducted in Iran are mainly limited to the study of essential oil components. Scope: Considering biological effects reported from this genus, purification and analysis of compounds present in Teucrium orientale, seems rational. Methods and materials: The methanolic extract previously prepared during the previous dissertation process is suspended in distilled water and then extracted from water-saturated ethyl acetate and n-butanol solvents, respectively. In this way, methanolic extract is converted to ethyl acetate, butanol and aquic fractions. The resulting fractions will be separated by VLC. The resulting pure compounds will be identified by proton and carbon NMR. Results: A flavonoid compound known as Isorhamnetin 3-glucoside was identified in this investigation. Conclusion: The biological effects of this genus is probably due to its poly phenolic derivates.