Determination of immunohistochemistry staining scale of C4d for diagnosis of Membranous Glumronephritis Patients

Abstract

Membranous glomerulonephritis is the most common couse of adult’s nephrotic syndrom. Diagnosis of membranous nephritis is based on light electron immuuno fluorescence microscopy and clinical signs. Immune complex deposition , against podocyte antigens such az PLA2R activate complement system.C4d is a complement’s products in classic and lection pathway.This marker can be used by immunohistochemistry to diagnose MGN when other methods are not available. Methods: In cross sectional study in Tabriz medical university, pathology department,33 patients with primary membranous glomerulonephritis and 20 patients with minimal change disease, which diagnosis was confrmed by light microscopy and immunofluorescence included in the stady as target and control groups. C4d immunohistochemical statning was performed for both groups and staining of glomerular capillaries was comared between two groups. Result: 53 patients included in this study.There was no significant demographiics difference between the two groups.(except age: Pvalue 0.002) C4d immunohistochemical staining was positive in glomerular capillary (2+ to 4+) in most case of MGN but was negative is MCD patients. Sensivity and specifity of C4d IHC Staining are 95% and 100%. Pearson correlation between C4d(IHC) and IgG(IF) is 0.74 and between C4d(IHC) and C3(IF) is 0.65.