In-vivo evaluation of novel mucoadhesives nanoparticles containing dexamethasone for ocular drug delivery on rabbit eye

Abstract

Background and Objective: Ocular drug delivery is an extremely challenging area due to its restrictive barrier functionalities. Drug transport via corneal/non-corneal routes involves several intricate biological processes such as drug penetration across the ocular barriers and transfer to the anterior or posterior chambers, thus the purpose of this thesis was to investigate the in-vivo effects of a new smart polymer loaded with dexamethasone on inflammated rabbit eye. Methods: Polymeric smart micelles were prepared using N-isopropylacrylamide (NIPMAAM), vinyl pyrrolidone (VP) and methacrylate (MAA) as monomers which were cross-linked with N, N-ethylene bis-acrylamide (MBA) with 75.7:9.5:14.8 molar ratios. These micelles were further characterized upon their physicochemical properties using particle size analyzer, FT-IR, H-/C-NMR,. Particle size analyzer resulted in 415-942 nm, when cross linker was MBA and 297-464 nm while applied cross linker was Triethyleneglykol. Then prepared nanosuspension dropped in rabbit eye according certain time courser, later results classified via Hogan's scoring courser and statically analyzed using Independent student t-test and MannWhitney U-tests. Results and Conclusion : The ocular barriers are highly specialized and selectively control the inward/outward traverse of compounds, so based on our study this novel copolymer loaded with dexamethasone as a novel drug delivery system due to its mucoadhesives and thermosensitive properties that prolongs residence time of medicine in target site can increases bioavailability of the model medicine and treats damaged rabbit eyes faster and better than simple dexamethasone drop available in the market.Key words: Ocular drug delivery, mucoadhesives, nano particle, N-isopropylacrylamide, smart polymer, dexamethasone, rabbit.