Evaluation of serum levels of gene indicators microRNAs as new diagnostic biomarkers in osteoporosis patients with Intertrochanteric fracture.

Abstract

Osteoporosis (OS) is known as the most common age-related bone disorder. Indeed, OS is a systemic skeletal disease associated with decreased bone mass and bone tissue destruction, leading to an increase in bone fractures. Intertrochanteric fractures are considered a relatively common extracapsular fracture of the femur during OS, which causes significant pain and severe disability that ultimately leads to a diminution in the quality of life. Therefore, trying to provide earlier and more accurate osteoporosis diagnostic methods is a continuous and appropriate effort. Serum-specific markers appear to be of high diagnostic value. Hence, microRNAs (miRNAs / miRs) are more sensitive biomarkers that have emerged as the central post-transcriptional modulators in bone development and homeostasis. It has been shown that miRNAs can play an essential role in differentiation, development, validation, and therefore as biomarkers for diagnosis. Methods: According to the recent study inclusion and exclusion criteria, 50 blood samples of patients with osteoporosis and 50 blood samples from healthy individuals were selected. After describing the importance of the present study and completing the written consent form by patients, their blood samples was collected and then serums were separated. Following RNA extraction and cDNA synthesis, the miRNAs expression profiles were amplified and measured using specific primers. Finally, the relationship between miRNAs in serum samples was compared between patients' two statistical populations and healthy. Results: Our results illustrated that mRNA expression levels of miR-125b (*P< 0.05), miR-122 (**P< 0.01), has-miR129-5p (**P< 0.01), has-miR-146a (**P< 0.01) and has-miR-1219 (**P< 0.01) significantly up-regulated in osteoporotic patients with intertrochanteric fractures compared with control group.