Correlation (Linking) of gastrointestinal flora with inflammatory markers in patients on maintenance hemodialysis

Abstract

Inflammation occurs when the intestinal microbiota alters and can stimulate the translocation of uremic toxins into the systemic circulation. In this trial, we assessed differences in microbiota compositions, serum levels of inflammatory cytokines and bacterially derived uremic toxins between the patients with End-stage renal disease (ESRD). Methods: Stool and blood samples of 20 patients were collected. DNA genome of bacterial composition of stool samples was extracted and evaluated by sequencing analysis of 16S rRNA genes. Related methods were used to measure the serum levels of inflammatory cytokines and uremic toxins. Results: The mean of serum concentration of TNF-α, IL1-β, IL6-β, indoxyl sulfate and p-cresyl sulfate were measured 306, 2225, 160 ng/ml, 36.76 and 0.392 μM/mL among 20 ESRD patients, respectively. The bacterial composition demonstrated that the abundance of family Bacteroidaceae, Bifidobacteriaceae, Lactobacillaceae and Prevotellaceae was 4.30%, 3.95%, 3.41% and 1.73%, respectively. We were observed significant strongest positive correlation between family Prevotellaceae and TAS, Lactobacilli species and CRP and PCS, S. wiggsiae and IS (P<0.001). We were only found strongest negative correlation between B. clarus and PCS.