Evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis

Abstract

Background: Contamination of bloodstream by microorganisms or their toxin is called sepsis. Severe oxidative stress is an important event that occurs in patients with sepsis. The body has extensive and multiple defense mechanisms against the reactive oxygen species (ROS) produced during inflammation and sepsis; however, there are many notable evidences for redox imbalance and oxidative stress in sepsis. Gamma Oryzanol (GO) is one of major bioactive components in rice bran, and has been considered to function as an antioxidant. Objective: This study was aimed to evaluate the effect of GO on oxidative stress factors of septic rats. Methods: To induce sepsis, caecal ligation and puncture (CLP) method was applied to rats. 50 Male Wistar rats were divided into following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group; 100 mg/kg GO- treated CLP group and 100 µg/kg Selenium- treated CLP group. GO was administered with an oral gavage 2 hours before CLP and Selenium was injected i.p. 0.5 hours after CLP. Blood and tissue samples were collected 12 hours after CLP to assay oxidative stress biomarkers and prepare tissue section for histopathological studies. One of the lungs of each rat was frozen in liquid nitrogen and kept at -70°C for enzymatic activity analysis and the other was kept in formalin 10% until tissue section preparation performed for histopathological studies. Results: Plasma total antioxidant capacity (TAC) level was increased in GO- treated CLP groups. Myeloperoxidase (MPO) activity was significantly lower in GO 50 mg/kg- treated CLP group and Selenium- treated CLP group. Glutathione peroxidise (GPx) level was improved by treatment with GO 100 mg/kg and Selenium 100 µg/kg. Conclusion: It seems that GO has protective effect on lung inflammation and improves body redox capacity during acute sepsis induced by CLP.