The investigation of the effects of Carvacrol and Minocycline on spermatogenesis and biochemical blood factors in the diabetic model of adult rats

Abstract

The aim of this study was to investigate the effects of carvacrol and minocycline on spermatogenesis and blood biochemical factors in adult diabetic rats. Materials & Methods: Male rats (n=72) were randomly divided into 9 groups. Control group, diabetes, healthy treated with citrate buffer, diabetic treated with carvacrol (75 mg/kg), diabetic treated with minocycline (100 mg/kg), diabetic treated with carvacrol+minocycline, healthy treated with carvacrol, healthy treated with minocycline, healthy treated with carvacrol+minocycline. After the treatment period (8 weeks), plasma levels of oxidative stress markers (SOD, GPX, and MDA) were measured and the expression of Bax and Bcl-2 genes were assessed by RT-qPCR. Sperm parameters and hormone levels (LH, FSH, and testosterone) were also examined. Results: Johnson score along with epithelial thickness and seminiferous tubule diameter increased in the diabetic group after the treatment with carvacrol and minocycline compared with the diabetic group. The levels of testosterone, LH, FSH and antioxidant enzymes in the treatment groups increased significantly compared to the diabetic control group. MDA levels and Bax expression showed a significant decrease in the treatment groups. Bcl-2 expression was also increased in the treated groups.