Evaluation of prophylactic effects of Olanzapine and Honey on neuropathic pain induced by Cisplatin in mice

Abstract

Introduction: In the process of neuropathy induced by anticancer drugs, several mechanisms have been mentioned such as activation of the oxidative stress system, increasing the activity of NMDA receptors, and serotonin receptors. Neuropathic pain is a result of various mechanisms operating at the peripheral, spinal cord and supra-spinal levels, which cause alterations in the pain conduction pathway. This may also develop secondary to some other pathological conditions such as diabetes mellitus, cancer, herpes infection, autoimmune diseases and HIV infection etc. Goal: The aim of the current study was to evaluate the preventive effects of olanzapine and honey on cisplatin-induced neuropathic pain that was the result of cisplatin in the (spurious) mice. Materials and methods: 81 male mice in a weight range of 25 to 35 grams were randomly divided into 9 equal groups and underwent olanzapine and honey for 25 days. Different doses of olanzapine and honey were injected three days before injection of cisplatin . at the fourth day we inject cisplatin (5 mg/kg,IP ). Then injection of olanzapine and honey continued for 3 days. After that we have 3 days for rest. Finally we performed hot plate test. This 7 day cycle repeated 3 times for all groups. At day 25, blood samples were collected for MDA and TAC estimation. Result: The results was showed a meaningful increase in reaction to the pain after injection of these doses (2.5, 5, 10 mg/kg, IP) of olanzapine and honey (2,4 mg/kg, IP) and also was observed the meaningful impact of olanzapine and honey in these doses can decrease MDA and increase TAC level. Also, co-injection of the minimum doses of this drug had a statistically better effect than injection of each of them alone. Conclusion: It is probable that a part of the inhibitory effects of olanzapine and honey is mediated by the control of oxidavtive stress. At the same time, we suggest further studies to obtain precise mechanisms.