Screening and optimization of Halophilic bacteria producing phytase and beta-glucanases from center of Iran

Abstract

In some areas, there are very difficult conditions for life that appear to be life-free at first. Those areas have very high salinity condition, while this salinity is a good environment for halophilic microorganisms, these microorganisms have the ability to produce hydrolytic enzymes. Some of these enzymes are Phytase and beta-glucanases. Phytase is an enzyme essential for bone health and digestive system, which is widely used in the paper industry, food industry, fruit juice industry, animal feed and poultry and medicine. Studies confirm that the use of this enzyme reduce the body needs to calcium and phosphate. Also, beta-glucanases is a very important enzyme as the body cannot make it itself. The capability of this enzyme to digest fiber helps to overcome gastrointestinal problems such as mal absorption. This enzyme has various uses in the pharmaceutical, food industry, and so forth. Objective: The aim of this study was finding halophilic bacteria isolated from halophilic soil of center of Iran producing Phytase and beta-glucanases enzymes and optimizing their growth. Materials and methods: At first, the halophilic bacteria were screened for producing of Phytase and beta-glucanases enzymes. Then, pH and temperature were optimized for the best growth of bacteria. Results: Results showed that two out of seven isolated halophilic bacteria were positive for beta-glucanases activity but none of them could produce phytase. The halophile producing beta-glucanases belong to Bacillus genus. Also, the best pH and temperature for the growth were 9 and 50 °C, respectively.

Conclusion: Isolated Halophilic bacteria from the deserts of central Iran have the ability to produce hydrolytic enzymes and so that it can be the good source of hydrolytic enzymes such as phytase and beta-glucanases for future research and industrial work.