Design and preparation of phage-based nano-biosensor for measuring ROR-1 protein

Abstract

The ROR-1 receptor expression in many cancers, such as colorectal cancer, blood, kidney, breast and many other cancers, as well as the role of this receptor in metastasis and the progression of cancer cells, this proto-oncogenes can be measured by methods. Sensitive found the necessary prognosis and prevented the development of cancers. To date, ROR-1 measurements have been performed with the ELISA, Western Bloat, and Real time PCR techniques, but alternative methods such as biosensors can be used to measure more sensitivity as well as save time and cost-benefit. Recently, biosensors have become common analytical tools for small analyzes due to their high sensitivity and wide analytical range. In the present work, the development of a new biometric method based on the Graphene quantum dots (GQD) and Silver nanoparticles has been introduced for rapid and selective detection of ROR-1 protein. Bacteriophage-based bio measurers of M13 were used as a marker of leukemia for electrochemical detection of ROR-1 protein. Bacteriophage M13 (virus), as a biological cognitive element, was placed on glassy carbon electrodes modified on the basis of Graphene quantum dots and silver nanoparticles. The presence of each of these factors on glassy carbon electrodes was confirmed using scanning electron microscopy (SEM) and square wave voltammetry (SWV). The biosensor designed to measure the amount of ROR-1 protein was used in standard solutions, resulting in an optimal detection limit of one picograms per milliliter. Finally, this expanded platform was used to evaluate ROR-1 protein in serum samples of patients with CLL, and the results obtained from the biosensor were compared with the results obtained from the Eliza kit. As an interesting part of this study, some of the concentrations of ROR-1 protein in the samples of patients with CLL who were in the early stages of the disease, which could not be identified by Eliza, but, were easily analyzed by this biosensor.