Evaluation the expression level of miR-130a and miR-615-3p involved in asparagine metabolism in tumor and margin tissue of patients with breast cancer

Abstract

Breast cancer (BC) is the most common malignancy among women with high mortality rate. The blockade of asparagine-related pathways may be an effective measurement to control the progression and reduction of BC metastasis potential. Recently, it has been shown that various miRNAs as part of small non-coding RNAs have a great role in cancer development, especially the asparagine-related pathways, to modulate invasiveness. This study aimed to evaluate the expression of miR-130a-5p and miR-615-3p in tumoral and non-tumoral adjacent tissues of patients with breast cancer. Methods: There is a chance that asparagine metabolism is influenced by miR-130a-5p and miR-615-3p confirmed by bioinformatics analysis. Hence, real-time PCR conducted on eighty BC tumoral and non-tumoral adjacent tissues to evaluate the expression level of the two miRNAs. In order to predict the potential biological process and molecular pathways of miR-130a-5p in-silico analysis was applied. Results: This study demonstrated that miR-130a was downregulated in tumoral tissues compared to non-tumoral adjacent tissues (P-value= 0.01443 and fold change= -2.5137). On the other hand, miR-615-3p did not show any significant difference between the two groups. Furthermore, the subgroup studies did not reveal any significant correlation between the expression of these two miRNAs and subfactors. Furthermore, in-silico studies unraveled several biological processes related to amino-acid metabolism, as well as pathways related to tumor development such as Phosphatase and Tensin Homolog (PTEN) and JAK-STAT pathways among miR-130a-5p target genes.