Study of the anti-proliferative and anti-migratory response of endothelial cells to the treatment with Docetaxel anti-cancer agent

Abstract

Genetic changes play an important role in the development of gastric cancer. In addition to changes in the level of DNA, changes in the expression level of MicroRNAs are also important in cancer progression. It is understood that the intercommunication between cancer cells and their microenvironment is essential to tumor angiogenesis. Exosomes are extracellular vesicles that are important mediators of intercellular communication and play a role in promoting angiogenesis. However, very little is known about the contribution of breast cancer exosomes to tumor angiogenesis or whether exosomes can mediate docetaxel (DTX)’s anticancer action. Methods: HUVEC cell line was cultured in complete RPMI-1640. Cells were treated for 24 hours with DTX, CoCl2, DTX and CoCl2. Cells were harvested, and RNA extraction and cDNA synthesis were performed from cells and exosomes using standard methods. Expression levels of miRNAs and their pro-angiogenic target genes were analyzed using quantitative real-time PCR (qRT-PCR). Results: We observed a decrease in the expression of pro-angiogenic gene including VEGF in cells after treatment with DHA in normoxic and hypoxic condition. And also the expression of tumor suppressor miRs including miR-16, miR-152 was altered by DTX in treated cells.