Evaluation of Eosinophils in Odontogenic Cysts

Abstract

Introduction: Considering the possible role of eosinophils in the pathogenesis of odontogenic cysts, further understanding of the role of these cells can play a role in controlling the spread and recurrence of the disease and ultimately to create more effective treatment strategies, reduce patient morbidity and reduce costs. Lead to complications and recurrence of the disease. Considering the lack of sufficient studies in this field, the aim of current study was to evaluate the level of eosinophils in three types of radicular cysts, dentigerous and keratocysts using Congo-Red staining and also to investigate the effect of age and sex on levels of eosinophils. Methods and Materials: This descriptive-analytical cross-sectional study was performed on 45 cases of paraffin blocks of odontogenic cysts including 15 radicular cysts, 15 dentigerous cysts and 15 keratocysts, which were randomly selected from the archive of the pathology laboratory of Tabriz Dental School. Samples were stained with 1% Congo-red solution and the total density of eosinophils was examined in 10 microscopic fields with a magnification of 40 by an oral pathologist. The characteristics of the cysts along with the demographic characteristics of the respective patients were recorded and examined. Results: In this study, the mean of eosinophils in dentigeros cysts was 1±0.2, in radicular cysts was 1.5±0.3 and in keratocysts was 1.1±0.1. Comparing the means of eosinophils between odontogenic cysts showed no significant difference between dentigerous, radicular and keratocyst cysts. In none of the cyst types, a significant relationship between age and sex of patients with the mean of eosinophils was obsereved. Only for dentigerous cysts, the mean of eosinophils in the maxilla was significantly higher than the mandible, in other types of cysts, there was no significant relationship between the location of the cyst and the mean of eosinophils. Conclusion: Further studies are needed to investigate the possible role of eosinophils in the pathogenesis of odontogenic cysts and the effect of the use of inflammatory cell antagonists in treatment of cystic lesions.