Evaluation of level of expression of SOX-7 and MnSOD gene in tumoral and marginal lung tissues

Abstract

Lung cancer is the second most common cancer in the world. Genes involved in various cellular processes, such as the cell cycle, apoptosis, and cell migration, play an important role in the cancerous process in the colon. The MnSod and SOX-7 genes are two important genes that have been confirmed to play a role in cancer in previous studies. In this study, the change in the level of relative expression of these genes during carcinogenesis was analyzed and evaluated. Materials and Methods: During the surgical procedure in patients with lung cancer, a set of 50 tumor tissues and normal marginal tissue was collected. After extracting the total RNA, the Real-time PCR method was used to measure gene expression changes. Differences in mRNA expression levels of these genes, as well as changes in the expression expression of these genes in response to conventional therapies with appropriate statistical tests, were investigated. In statistical tests, P-value <0.05 was considered significant. Results: SOX-7 relative expression level in tumor tissues decreased significantly compared to healthy marginal tissues (P-value = 0.0018) On the other hand, the relative expression level of MnSod gene in tumor tissues increased compared to healthy tissue. Showed (P-value = 0.059), but this increase in expression was not significant in the samples in the specified confidence interval. In relation to the association of gene expression changes with the clinical and pathological features of patients, the only significant relationship observed was related to the relationship between SOX-7 expression and lymph node involvement (P-value = 0.0131). Also, in the case of MnSod gene, although the increase in expression of this gene was not significant in tumor and marginal samples, but the expression of this gene was related to STAGE (P-value = 0.015) and involvement in lymph nodes (P-value = 0.015). In fact, increasing the expression of this woman's gene has a bad prognosis for patients.