The Effect of Mesenchymal Stromal Cell-Derived Exosomes on Differentiation of Cord Blood-Derived Haematopoietic Stem Cells (CD34+) into Myeloid Cell Lineage

Abstract

In this study, the effect of MSC-derived exosomes on the differentiation of CD34+ HSCs from umbilical cord blood into the myeloid lineage investigated.Materials and methods: In this study, umbilical cord blood-derived CD34+ HSCs were cultured in the IMDM culture medium supplemented with 10% FBS in the presence of MSC-derived exosomes that isolated by ultracentrifugation technique. The culture of CD34+ HSCs performed in three groups: 1) Culture in the presence of G-CSF and IL-3 cytokines. 2) Culture in the presence of G-CSF and IL-3 cytokines and 10 μg/ml of exosomes concentration. 3) Culture in the presence of G-CSF and IL-3 cytokines and 20 μg/ml of exosomes concentration. Finally, by using the flow cytometry and Real Time-PCR techniques, CD34+ HSCs differentiation into myeloid lineage investigated.Results: The expression of myeloid lineage-specific markers (CD13 and CD33) in the presence of different concentration of exosomes was higher compared to the control group but increased expression of CD13 was not significant. In addition, the expression of specific genes of the myeloid lineage, including G-CSFR, PU.1, RUNX1, and C/EBP-α increased compared to the control group, which the increase in the expression of G-CSFR, C/EBP-α, and RUNX1 genes was statistically significant (P <0.05), but the increase in the expression of PU.1 gene was not statistically significant.