Dispersive solid‐phase extraction adsorbent of methamphetamine using in‐situ synthesized carbon‐based conductive polypyrrole nanocomposite: focus on clinical applications in human urine

Abstract

Methamphetamine consumption is a great concern in the society. Growing trend of methamphetamine abuse leads to crime among young people. Methamphetamine stimulates central nervous system and makes mental and physical disorders. Therefore, methamphetamine determination is a great interest among researchers in clinical laboratories. Aim Polymeric graphene oxide nanocomposite was synthesized and applied as new adsorbent in dispersive solid phase extraction (DSPE) of methamphetamine from human urine samples. Material and method Modified Hummer method was applied for synthesis of nano graphene oxide. Chemical method was applied for synthesis of polymeric nanocomposite. After full characterization of adsorbent by various techniques, the nano composite was applied as dispersive solid phase extraction adsorbent. Effective extraction parameters were optimized by one- parameter-at-a-time method. Analysis of real samples were carried out by the proposed method.

Results

Polymeric nanocomposites were used for efficient extraction of methamphetamine. The synthesis method of polymeric nanoadsorbents was easy and reproducible responses were achieved in methamphetamine extraction. Polymeric nano adsorbents were characterized by different techniques such as IR, XRD and SEM and then optimization of effective parameters in the extraction process were performed. The calibration curve was plotted in the concentration range of 300-800 ng/mL and validation of the method was carried out. Limit of the detection was 9 ng/mL.

Discussion

The analysis of methamphetamine in addicted subject was carried out after DSPE and determination with the proposed method confirmed the capability of the proposed method in different laboratories.