Evaluatin of polymorphism of ABCA7, TREM2, EPHA1 MS4A6A and CD33 genes in patients with late onset Alzheimer’s disease

Abstract

Introduction: Late-onset Alzheimer's disease is an age-related neurodegenerative disease that occurs after age 60-65 and is the most common form of Alzheimer. The causes of late-onset Alzheimer are still not fully understood, but the combination of genetic and environmental factors contribute to the disease development. Detection of new loci that affect the risk of late-onset Alzheimer's disease are important in understanding the etiology of this disease. Genes that are associated with the risk of late-onset Alzheimer's include: APO E, ABCA7, CLU, CR1, CD33, CD2AP, EPHA1, BIN1, PICALM, MS4A, TREM2 and PLD3. Materials and Methods: This study was the case - control study. Two group were Azeri breed and 10 years old and up. 100 patients and 89controls were investigated for the polymorphism of genes ABCA7, CD33, TREM2, EPHA1, and MS4A6A. All of volunteers were sampled. Then DNA of samples extracted by phenol -chloroform method. Finally, set up the RFLP method to determine polymorphism.

Results: Regarding the results of electrophoresis, between two groups of patients and control in terms of distribution of heterozygote and homozygote genotypes CD33 (rs3865444), there was a significant difference in homozygous states GG (pvalue 0.001) ,and heterozygotes(pvalue 0.000). In the case of polymorphisms TREM2 (rs75932628), ABCA7 (rs3764650), MS4A6A (r8983392 and EPHA1 (rs11771145), the difference between the two groups was almost the same distribution and not significant, and in all cases, heterozygotes and homozygotes were compared between the two groups have been P value> 0.05.