Effect of troxerutin on expression of miRNA192, TGF-β and SIP1 levels of kidney tissue in type 1 diabetic male rats

Abstract

Nephropathy is one of the most important complications of diabetes. The increase of TGF-β and miRNA192 levels in diabetic conditions, by suppressing SIP1, increases the level of collagen in the kidney tissue and leads to nephropathy. So, in this study we inspected the effect of troxerutin on cellular processes of nephropathy in type1 diabetes, from the aspect of the miRNA and its related pathways. Materials and methods: Fifty male wistar rats (200-250 g) were randomly divided into 5 groups (n=10): control, sheme under treatment with troxerutin (150 mg/Kg/day/PO) for 4 weeks. Diabetic (induced by STZ 55 mg/kg, IP, single dose). Diabetic+ Insulin, under treatment with insulin, (4-6 units/day, NPH) for 4 weeks. Diabetic+Troxerutin. At the end, the kidney tissue of all animals were extracted for the measurement of miRNA192, SIP1 and TGF-β. Proteins and miRNA were measured by ELISA and Real time PCR respectively. Results: Diabetes significantly increased TGF-beta and miRNA192, but troxerutin and insulin decreased them(p<0.05). On the other hand, diabetes significantly reduced SIP1 and troxerutin increased it(p<0.05), but no significant difference was seen between insulin and troxerutin.