Investigating the effect of substitution of Acellular Wharton's jelly for FBS on the myloid and lymphoid lineage specific markers and proliferation pattern of hematopoietic stem cells (CD34+) following co-culture with mesenchymal stem cells

Abstract

In this study, we investigated the effect of substitution of Acellular Wharton's jelly for FBS on the myeloid and lymphoid lineage specific markers and proliferation pattern of CD34+ cells following co-culture with Mesenchymal Stem Cells. Materials and methods: In this study, umbilical cord blood HSCs CD34+were co-culture with MSCs in the presence of SCF, TPO, and FLT3-L. HSCs CD34 +were cultured in two groups: 1) IMDM medium containing 10% FBS 2) IMDM medium containing 10% AWJ. Finally, amplification pattern and expression of myeloid and lymphoid lineage markers were evaluated using flow cytometry and Real Time-PCR methods. Results: The expression of specific myeloid markers including CD14, CD15 and CD33 was increased in the culture medium containing Acellular Wharton's jelly compared to the FBS group, which only increased CD33 significantly. Also, expression of myeloid-specific genes including C/EBP-α, RUNX1 and PU.1 in Acellular Wharton's jelly group were increased compared to FBS group, which as significantly increased in expression of C/EBP-α and RUNX1 genes, but The increase in PU.1 gene expression was not significant. In addition, expression of specific markers of lymphoid lineage including CD19, CD3 in the culture medium containing Acellular Wharton's jelly was decrease compared to FBS group, which was significantly decreased in CD3. In addition, expression of specific lymphoid lineage genes including IKUROS, GATA3 in Acellular Wharton's jelly group was decrease compared to FBS group, which was significantly decreased in GATA3 gene expression.