Comparison of the effects of Bupropion and Celecoxib in behaviral and inflammatory parameteres in dependency induced by Morphine in mice

Abstract

One of the problems associated with long-term use of morphine is the effect of tolerance and dependence, on the acute use of morphine affecting the production and secretion of various cytokines (TNF-α, IL-β, etc.) and the glutamate system. Bupropion, an antidepressant, and celecoxib, as an anti-inflammatory drug, affects the secretion of various cytokines. Objective: In this study, the possible effects of bupropion and celecoxib on morphine-induced dependence were evaluated. Materials and methods: In the present study, 90 male mice (20-30 g) were divided into 10 groups of 9 mice. Different groups received the drug regimens for 6 days, respectively. The animals were given different doses of bupropion (2, 4, 8 mg / kg, i.p.) or celecoxib (5, 10, 20 mg / kg, i.p.) for two days individually or in combination (bupropion 2 mg / kg, i.p and celecoxib 5 mg / kg, i.p.). The mice were recived morphine (50mg / kg, i.p.) for four days, along with doses of the studied drug regimens. Animals received naloxone (4 mg / kg, i.p.) after 2h of the last dose of morphine, and symptoms of discontinuation (jumping and standing on feet) were assessed for 30 minute in each animal. Results: The results showed that the administration of different doses of celecoxib had no significant effect on morphine withdrawal symptoms. But bupropion at 8 mg/kg dose significantly increased withdrawal symptoms (P <0.001). Also, no significant changes in serum levels of inflammatory factors caused by different doses of the studied drugs were observed. Conclusion: Celecoxib and bupropion at the doses studied are not suitable candidates to reduce morphine-induced dependence.