Study on the effects of Feric nitrilotriacetate on 7, 12-Dimethylbez (a) anthracene and benzyoil peroxide induced skin tumorigenesis in the mice

Abstract

Cancer incidence is one of the human problems and in order to understand its mechanistic aspects several studies have been performed. Ferric nitrilotriacetete¬(Fe-NTA) causes tumors in the kidney. Objective: The purpose of this study was to investigate the carcinogenesis effect of Fe-NTA in mice skin. Methods¬&¬Materials: In first the back of the mice were shaved. After 24hours the materials were applied to the skin of mice, the mice 3¬doses of 6,¬9 and 12mg of Fe-NTA Topical tumor tumors of mice were examined locally on each rat for 15 days. After 9hours of the last dose of rats 7,12-dimethylbenzo(a) anthracene received 60(μg). Then, they were exposed to benzoyl peroxide 20mg /200μl twice a week for 28 weeks. After 7 days, animals were promoted topically by banzoyl peroxide¬(BPO) twice weekly for period of 28¬weeks. Results: At the end of 10th week the first tumors were observed in the group of animals receiving ferric nitrilotriacetete 12¬mg, plus DMBA and promoted with BPO. However in the control group, the tumor occurred at end of the week 14. Also both number and percentage of tumor incidence significantly were higher in group receiving Fe-NTA 12¬mg at various time points. Further, in the tumor tissues the level of iron was higher in Fe-NTA¬(12 mg) treated animals. The results of this study may suggest that oxidative stress generated by iron overload is responsible for the BPO-mediated skin carcinogenesis.

Conclusion: The results obtained from this study showed that higher dose of Fe-NTA could exacerbate skin tumors in comparison to the control group. Overall, our study demonstrates that Fe-NTA in its high dose is responsible for mouse skin carcinogenesis. The role of excessive doses of Fe-NTA in mice skin induction may be due to its potential capabilities of producing oxidative injuries and DNA damage.