Comparison of the levels of salivary HEAT SHOCK PROTEIN 70 (HSP_70) in chronic periodontitis with healthy individuals

Abstract

Intrudoction: Chronic Periodontitis is an inflammatory disease,one of the important sign is attachment loss of gingival and periodontal fiber. Studies show that HSP plays an important role in etiology of chronic periodontitis. Heat shock protein(HSPs) are a group of intra-cellular proteins that play scavenger role for other intra-cellular proteins under denaturation stress conditions.HSP70 may be as a pro-inflammatory cytokine that causing tissue damage in periodontitis. Substantial studies were measured and used the sera of the patients,however non of them focused on the HSP70 levels in the saliva as a biomarker.also taking samples of saliva in comparison with the supply of their sera,is a non-invasive for the patients and ethically acceptable in research community. There for,the aim of this study,is to evaluate the HSP-70 level in saliva of patient as a diagnostic criteria for chronic periodontitis. Materials and methods: in this case-control study,after taking samples from saliva of chronic periodontitis and healthy individual,during that periods,HSP-70 levels,were evaluated by ELISA method and HANGZHOU kit. SPSS 16 was used to carry out descriptive statistics analysis and ANOVA and Tukey test analysis were used.P value is calculated and less than 0.05 was considered significant in this study. Result: in this study,45 cases of chronic oeriodontitis and 45 cases of healthy individuals, who referred to dentistry faculty in Tabriz,iran since march 2019 to july 2019 were evaluated. Conclusion: Results of this study showed that HSP-70 levels in patients' saliva are higher than HSP-70 levels in healthy individuals' saliva.