Evalution of HSP70 level in patients' saliva with Oral lichen planus disease, in comparation with Healthy individuals

Abstract

Introduction: Oral lichen planus is an inflammatory disease. the need for biopsy to get an accurate diagnosis of oral lichen planus is discussed, but there are still no clear guidelines that is universally approved. Studies show that HSPs plays an important role in etiology of lichen planus. Heat Shock Proteins (HSPs) are a group of intra-cellular proteins that play scavenger role for other intra-cellular proteins under denaturation stress conditions. HSP70 maybe as a pro-inflammatory cytokine that causing tissue damage in Oral lichen planus. Substantial studies had been carried out in the field of lichen planus disease and majority of them were measured and used the sera of the patients, however, none of them focused on HSP70 levels in the saliva of OLP as a biomarker. Also taking samples of saliva in comparison with the supply of their sera, is a non-invasive for the patients and ethically acceptable in research community. There for, the aim of this study, is to evaluate the HSP70 level in saliva of patient as a diagnostic criteria for OLP. Materials and Methods: In this case-control study, after taking samples from saliva of lichen planus patients and healthy individuals, during that periods, HSP70 levels, were evaluated by ELISA method and HANGZHOU kit. SPSS 17 was used to carry out descriptive statistics analysis and T-test and ANOVA analysis for independent samples. P value is calculated and less than 0.05 was considered significant in this study. Results: In this study,45 cases of not-cured lichen planus disease patients with oral lesions and 45 cases of healthy individuals, who referred to dentistry faculty in Tabriz, Iran since January 2019 to July 2019 were evaluated. The mean of HSP70 levels in patients' saliva was (20.37±6.75 ng/ml) and mean of HSP70 levels in health control group's saliva was (17.19±8.27 ng/ml). (P<0.05) Conclusion: Results of this study showed that HSP70 levels in patients' saliva with oral lesions of lichen planus disease, are higher than HSP70 levels in healthy individuals' saliva. This is the first report that evaluate and compare HSP70 level in saliva of the patients with OLP and healthy controls.