Comparison of HA class II correlation in ulcerative colitis patients with and without extra- intestinal involvement

Abstract

Many studies have shown that the major cause of gene mutation that is associated with an increasing in the incidence of ulcerative colitis is the short arm of chromosome 6, which is related to the HLA locus. Considering the significant role played by human leucocyte antigen (HLA) class II genes, it is necessary to investigate the correlation of this gene and UC extracellular complications. Therefore, in this study, we evaluated the correlation between different genotypes of HLA Class II and extracellular complications of ulcerative colitis. Materials and Methods: This is a cross-sectional study, in which 52 patients with extra-intestinal complication in ulcerative colitis and 51 patients with non-inferior ulcerative colitis as the control group was used. At first, demographic and clinical characteristics were taken from all individuals. Blood samples were collected from all samples, followed by DNA extraction and all specimens were immediately stored in a -70 C temperature. Finally, all specimens were analyzed by PCR-SSP for allele type and genotype related to DRB1 and DQB1. After collecting data, raw data was analyzed using SPSS version 22 software. Data was presented as descriptive statistics (mean ± SD for quantitative and qualitative data for qualitative data). Results: The highest frequency of DRB1 was observed in patients with extra-intestinal complications associated with DRB1 * 04 12 (11.3%) and in patients without complications DRB1 * 13.17 (19.31%). Also, the results of this study in DQB alleles indicated that the highest frequency was observed in patients with extra-intestinal complications associated with DQB1 * 05 and DQB1 * 05: 04 34 (35.78%) and in patients without complications was DQB1 * 06 33 (77 / 41%). In addition, the results of the association between DQB alleles in patients with and without extra-intestinal complications showed that this association was significantly different in DQB1 * 02 and DQB1 * 05 DQB1 * 05: 04 / and DQB1 * 06 alleles, respectively with P-value = 0.03 and P-value = 0.008 and P-value = 0.01 respectively.