preparation of anti-EGFR scFv-PE40 exotoxin and evaluation of its cytotoxicity on EGF expressing tumoral cells

Abstract

PCR and restriction digestion verified the integrity of the immunotoxin construct. Purification by affinity column resulted in a highly purified recombinant immunotoxin. MTT assay revealed the growth inhibitory effect of huscFv-PE40 immunotoxin on EGFR-overexpressing A431 cells with an IC50 value of 250 ng/ml.