Investigation of Hypoglycemic Effects of Zygoascus Hellenicus Yeast on Healthy and Diabetic Mice

Abstract

Introduction:Diabetes is the fifth cause of death in the world. different types of compositions in natural resources have been proposed to have medicinal effects on cellular targets. Objective:the aim of the present study is to investigate Zygoascus hellenicus yeast on blood glucose level in Syrian mice. Materials and Methods:Syrian mice(weighted 30-35gr)were randomly divided into two main groups of healthy and diabetic. Then the animals received 150mg/kg Streptozocin(solved in 0.1M citrate buffer with pH=4) in single dosage and peritoneal injection manner. Healthy control animal received normal saline instead of soluble Streptococin. Three days later blood glucose was measured by one drop of the animals total tail blood using glocometer. The Syrian mice with blood glucose above 11.1 mmol/L (200mg/dI) were considered as diabetic. After recording the animals weight they were divided into four groups, each group involving 10 members: 1-Healthy control group receiving distilled water, 2-Healthy control group receiving from yeast culture media 3-Diabetic control group receiving distilled water and 4-Diabetic group receiving from the yeast culture media. The then mice were treated twice a day by gavage method for two weeks. Results:Standard deviation (±)of blood initial glucose was 135.86 ±10.78 mg/dl, this value changed to 377mg/dl three days after Streptozocin injection and then it was measured three days after receiving blood glucose yeast, which has been decreased to 262.43±108.62mg/dl. Moreover in the Diabetic mice, which received distilled water, it reached to 425±103.24 mg/dl three days after Streptozocin injection and the value reached to 404.13±146.28mg/dl three days after receiving yeast and a significant difference was observed between the two groups (p>0.05). Conclusion:the use of Zygoascus hellenicus yeast resulted in decreased blood glucose in diabetic mice in animal studies. This finding can be related to effects of the yeast on insulin resistance and its reduction