Evaluationof mir-194،mir-106b and talin2،MMP2 expression in breast cancer cell lines, MDA-MB-231 after treatment with DHA

Abstract

In this study, we aimed to investigate the effect of DHA fatty acid on the expression of matrix metalloproteinase (MMP)-9, MMP-2, vimentin and talin2 genes, and tumor-suppressor mir-194 and onco-mir-106b, and their possible combination effect with taxol. Materials and Methods: Breast cell lines were cultured and MTT assay was performed. After RNA extraction and cDNA synthesis, the expression level of (MMP)-9, MMP-2, vimentin and talin2 genes, and mir-194 and, mir-106b,, were determined by quantitative real-time PCR for both treated and untreated cell lines under normoxi and hypoxic condition.. Also the result of western blotting for talin2 confirmed its real-time result under normoxi and hypoxic condition. Results: DHA and taxol, together or alone, inhibited the growth of breast cancer cell line in vitro. We also observed that the DHA treatment decreased metastasis and migration in a dose- and time-dependent manner in the MDA-MB-231 human breast cancer cell line. Our results demonstrated that DHA produced a significant effect by inhibiting MMPs, especially MMP2 and MMP9; HIF-1α and cytoskeletal proteins, such as vimentin. Moreover, taxol-DHA can influence the expression of the talin2 protein. combination of taxol and DHA significantly increased the expression of talin2. The control and treated cells subjected to western blot analysis to measure the talin2 protein level. DHA increases talin2 protein levels in breast cancer cells. Our results showed that after treatment with DHA, DHA-taxol increased expression levels of miR-194 and decreased miR-106b in MDA-MB-231.