Evaluating antifungal effect of Matrica and Zingiber on oral Candida Albicans species isolated from denture stomatitis outpatients referring to Dental Faculty of Tabriz University of Medical Sciences (in vitro)

Abstract

Background: Fungal infections are common oral disorders. For treatment of these infections, antifungal medicines have been developed with different formulations. Regarding the complications and problems with conventional drugs, the researchers seek to obtain effective drugs without side effects for treatment of this disease. In this regard, special attention has been paid to medicinal plants. The aim of this study was to investigate the antifungal effects of Salvadora persica, Mentha spicata and Achillea millefolium plants against candida albicans isolated from patients diagnosed with denture stomatitis. Materials and Methods: In this cross-sectional study, a sample of microbiological culture with sterile swab was taken from individuals referring to Tabriz dental faculty during a one-year period, which was examined by an oral pathologist and diagnosed with denture stomatitis. Sterile swab specimens were injected into the culture medium of TSB (Tryptic Soy Broth) and transferred to the Pharmacology Control Lab. The isolated isolates were grown in TSB medium and the standard inoculum was used to test the sensitivity determination by identifying the MIC(Minimum Inhibitory Concentration) of extract and essence of the plants. At the end of the MIC identification, the extracts and essences of the plants were compared with the MIC of nystatin and methanol from the samples. Data were analyzed using SPSS17 statistical software. In this study, P less than 0.05 was considered significant. Findings: There was a significant difference between the mean of MIC in the nystatin group with the zingiber extract group and matrica extract group and the mean of MIC in the nystatin group was lower than that of the two groups (P-value <0.05). There was a significant difference between the mean of MIC in the zingiber essence group with the mean of zingiber extract group and the mean of MIC in the essence of zingiber was lower than zingiber extract group (P - value <0.05)

Results: There was a significant difference between the zingiber extract group and nystatin, as well as and matrica extract group and nystatin in terms of effect on Candida albicans and both had an antifungal effect lower than nystatin. Also, in the same way, the use of zingiber essence is preferable to its extract. Regarding the effectiveness of nystatin on the control of Candida albicans, it can be concluded that the use of zingibe essence to study the inhibitory growth of the fungus and avoid denture stomatitis seems justified. Also, due to the benefits of herbal extracts and essences such as less complications, lack of resistance, better flavor and easier use for patients, the extracts of the zingiber and matrica at higher concentrations, can have an effect almost equivalent to nystatin.