screening and optimization of PH and temperature for producing of hydrolytic enzyme byzygoascus hellenicus

Abstract

Introduction Yeasts are eukaryotic microorganisms which classify as family of fungi. size of yeasts are highly variable that is related to type of species and environment that means wide range of yeasts are between 3 to 4 micron and some of them have grown until 40 micron. Zygoascus are belong to Trichomonascaceae family. It includes several species such as Zygoascus hellenicus and Zygoascus meyerae. Yeasts are able to produce hydrolytic enzymes so it is very important in industry. Hydrolytic enzyme such as amylase, protease, amidase, esterase and lipase have wide application in several parts for example food industry, medical sciences and chemical industry. Objective: In this research, producing of hydrolytic enzyme was studied by Zygoascus hellenicus. And also, the optimum activity and stability of the enzyme in several pH and temperature were studied. Materials and methods: Initially, the isolated Zygoascus hellenicus was cultured in a special yeast culture medium, then was studied for the production of hydrolytic enzymes such as amylase, protease, lipase, pectinase, cellulase and asparaginase. The best activity and stability of the produced lipase enzyme were studied at various pH and temperatures. Results: The results showed that the Isolated Zygoascus hellenicus was positive to produce lipase enzyme, but it was negative amylase, protease, pectinase, cellulase and asparaginase was negative. Also, the best pH for optimal activity of lipase was 7, the best temperature for optimal activity of the enzyme was 50 °C, the best pH for enzyme stability was 7, and the best temperature for isolated lipase enzyme was at 50 °C. Conclusion: The isolated Zygoascus hellenicus has the ability to produce lipase enzyme and it is suggested as good source for production of the enzymes for research and industrial purposes