Ultra-structural study by transmission electron microscopy: Effect of omega-3 on ovary cell organelles after experimental induced poly cystic ovary syndrome

Abstract

Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 4% to 8% of women in the age of procreation. For evaluation the protective effects of omega-3 polyunsaturated fatty acid on ovarian structure in experimental PCO induced by estradiol-valerat, this research was done.Materials and Methods: Wistar female rats (n=40) were allocated into four groups, one control (n=10) and three test groups (n=30), that one group received omega-3 (60 mg/rat/orally/daily), second and third groups were induced PCO by single injection of estradiol-valerate (16mg/ kg/ i.m), third group also received omega-3 (240 mg/kg) for 60 consequence days. Animals were kept in standard conditions. On day 60, the ovarian tissue of Rats in whole groups were removed and prepared for pathological analysis.Results: vacuolated area and rough endoplasmic reticulum expanded, de-granulated, disorganized were seen in PCO groups; however, these side effects decreased in the groups that received omega-3 significantly (p<0.05) in comparison to experiment groups and ovarian weights in PCO experimental decreased significantly (p<0.05).Conclusion: Results revealed that administration of omega-3 could significantly treat PCO. This suggested that polyunsaturated fatty acid could diminish negative side effects of PCO on ovary tissue. é 2014 / PMCARAS . All rights reserved.