The hemodynamic effect of HEMADO on ischemic-reperfusion injury of male rat isolated heart

Abstract

Objectives: Evaluating the effect of HEMADO on injuries resulted from Ischemia-reperfusion and related mechanisms in isolated male rat heart, is the main purpose of this study. Methods: 42 male Wistar rats (250-300g) divided to six groups, each has seven members (n=7) as follow: without ischemia, control, drug, ischemia with ethanol, blocker and drug-blocker. The animals are anesthetized by Ketamine and Xylazine. The hearts were quickly removed and mounted on Longendorff apparatus and perfused by Krebs-Henseleit solution under constant pressure and temperature of 37°C. After 20 minutes of stabilization, Ischemic groups are also received 40 minutes of global ischemia and 90 minutes of reperfusion. In drug group, the hearts were perfused 25 minutes with Hemadoenriched Krebs-Henseleit before the Ischemia. Minimum dose of HEMADO was 0.1 micro mole per liter, hence work on three different groups which were obtained from this study, was applied. Results: HEMADO increased LVDP and significantly caused to decrease end diastolic pressure of the left ventricle (P <0/05), and also increased dp/dt max. in the drug group, these effects of HEMADO meaningfully declined when used with blocker of Mitochondrial Potassium ATP Channels. Conclusion: The findings of this study indicated that Hemado could protect the heart against ischemia-reperfusion injury by enhancing the LVDP and decreasing the LVEDP. The cardioprotective effect of Hemado may be mediated in part by Mitochondrial Potassium ATP Channels.