Protection role of GnRH antagonist on cisplatin infertility side effects

Abstract

Bachground: One of the main factors which could have side effects in spermatogenesis is used in chemotherapy to treat cancer patients. It is proved that the agents affect on cells especially with high proliferation such as spermatocytes and kill them through apoptosis induction. Since gonadotropin hormones (FSH, LH) affect spermatogenesis and are suppressed by chemotherapy agents, so administration of GnRH Antagonist to protect the cells against its side effects is in researchers' interest. Present study tried to investigate protective effects of cetrorelix in suppression of side effects of cisplatin as chemotherapy agent on the germinal epithelium. Methods: In the present study 30 male mice were divided randomly into 3 groups: Control, Experimental 1 received 2.5 mg/kg cisplatin daily intraperitonealy and Expermental 2 received cisplatin and subcutaneously injection of 0.25 mg/kg cetrorelix. Cetrorelix injection initiated 1 week before cisplatin treatment and continued at second and third weeks. After 35 days of last injection, the testes were removed and processed for histological and apoptotic analysis according to routine protocols. Results: Histological findings of seminiferous tubules showed that in cisplatin receiving group, number of spermatogonia cells, thickness of germinal epithelium, seminiferous tubules diameter and spermatogenesis index rate (SI) were decreased. Also in some of the tubules only sertoli cells were observed. In addition, our study showed that number of apoptotic cells was increased in this group. Cetrorelix had prohibitive potential against side effects of cisplatin via morphology of seminiferous tubules and decline apoptotic cells. Conclusion: Cisplatin has adverse side effects on germinal epithelium by induction apoptosis and cetrorelix can protect spermatogenesis process from cisplatin adverse side effects. Copyright é 2013 by Tabriz University of Medical Sciences.