Polymorphism of exon 1,3 Bcl-10 gene in paraffin blocks with non hodgkin's lymphoma diagnosis and blood sample of ALL in northwest of Iran

Abstract

Background and Aims: Bcl10 is a gene that recently identifed in B-cell lymphomas of mucosa-associated lymphoid tissues. It has been suggested as a target for mutation in multiple types of tumours. The recently described Bcl10 gene has been suggested to be a major target gene for inactivation in a variety of human cancers. Methods: EDTA blood from 20 patient with ALL, 20 paraffin block with non hodgkin's lymphoma diagnosis and 45 blood sample as controls were collected and DNA was extracted. The gene was amplified with PCR and product for exon 1,3 of BCL-10 gene were sequenced. Results:after sequencing of PCR product of exon 1,3 there is n't any meaningful mutation in BCL-10 that could be caused diaease. Conclusion:mutation in BCL-10 could n't be caused of malignancy and extracted DNA of paraffinic block could be used as a source of cellular genomic matter in molecular studies.