Influence of electromagnetic fields of two phases square wave with low frequency on serum alt and ast levels and histochemistry of hepatocytes' Glycogen

Abstract

Long term exposition to wave generator and radiowaves, has been associated with external and internal disorders in people. Human liver contains a complex of paranchymic cells that perform different vital functions. Clarification of the effects of such waves on the liver function, seems quite interesting. In the present study, the amount of Glycogen storage in the liver lobules and cytoplasm of hepatocytes in addition to the level of ALT and AST enzyme of liver in serum were evaluated. 24 male Newzeland white rabbits allocated into 4 equal groups as follows: control group (No treatment) and 3 experimental groups which were exposed to waves with frequencyies of 1,10 and 100 HZS. AT the end of experiment, serum ALT and AST levels were assayed by using an auto analyzer device. Histopathological sections of liver were prepared of stained with PAS FOR DETERMINATION OF GLYCOGEN STORES. Results were analyzed using spss (version 9) and t test. No significant changes were observed in enzymes levels. In microscopic control samples, a lot of Glycogen was found in liver lobules. Offloading was moderate at frequency 1 (HZ). In 10 HZ frequency offloading was enhanced (P<0.05), however in frequency of 100 HZS no offloading was present. It was concluded that, waves with 1 to 10 Hzs frequency can decrease liver glycogen stores, with no significant effect on serum ALT and AST levels. This needs more research. é IDOSI Publications, 2010.