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Geno/cytotoxicty and apoptotic properties of phenolic compounds from the seeds of Dorema glabrum Fisch. C.A

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نمایش/بازکردن
BI-4-191.pdf (1.769Mb)
تاریخ
2014
نویسنده
Eskandani, M
Dadizadeh, E
Hamishehkar, H
Nazemiyeh, H
Barar, J
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نمایش پرونده کامل آیتم
چکیده
Introduction: Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anticancer potency of bioactive compounds from the seeds of Dorma glabrum. Methods: Methanol extract was subjected to phytochemical investigation using normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of isolated compounds on CAOV-4 cell line was evaluated. Furthermore, Annexin V/PI staining and comet assay were used to study genotoxicity of compounds. Results: Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and skimmin (3), were identified. MTT cytotoxicity assay showed growth inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and (3) with an IC50 of 99.7, 87.3 and 70.03 ?g/ml at 48 h, respectively. Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in the (1)-treated cells, while (2)-and (3)-treated cells necrosis/late apoptosis was dominant event. Single/double strands DNA breakages were observed by comet assay in all treatments. Conclusion: This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum. أ¯?آ½ 2014 The Author(s).
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http://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/54415
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