Effect of antioxidant supplements on in vitro maturation of bovine oocyte

Abstract

This study was aimed at determining the effect of acysteamine supplement during in vitro maturation of bovine oocytes. Cumulus-oocyte complexes (COCs) from abattoir ovaries were matured in vitro in Hepes- TCM 199 supplemented with 0.2 mM sodium pyruvate, 1 ?g/ml 17-?-estradiol, 10% fetal calf serum (FCS), 0.5 ?g/ml FSH and 0 (control), 100 or 500 ?mol/ml of cysteamine for 24 hours. When COCs matured in TCM 199 media with 500 ?mol/ml cysteamine, The rate of maturation were increased as compared with control group (P < 0.05). Also, the rates of degenerated oocytes in treatment groups lower than control group that it is not significant.